The mothers and supporters gathered in front of Delta Airlines ticket counters at 31 airports to express their outrage over the incident and raise public awareness about breastfeeding.
"I just don't understand why you have these pop stars walking around and showing everything and me sitting down and breastfeeding my child is considered offensive," Malissa Coy (32) told AFP as she breastfed her nine-month-old daughter at Washington National Airport, where more than two dozen other women gathered with their children.
The women, some sporting T-shirts and stickers bearing a breastfeeding symbol, held the "nurse-in" quietly for a little over an hour before leaving the airport as police and passengers looked on.
Similar "nurse-ins" were held at other airports across the country including in California, New York, Florida, Michigan, Arizona and New Mexico.
"Public policy is trying to encourage women to nurse but yet when women do that the message they are getting is that it is something shameful and dirty," said Lorrie Leigh, a mother of four who took part in the protest at the Baltimore-Washington International Airport in Maryland, where some 30 women gathered.
The incident that prompted the protests took place on October 13 and involved Emily Gillette (27) who was nursing her 22-month-old daughter before takeoff on board a Freedom Airlines flight that was three hours late.
A flight attendant for Freedom Airlines, a regional airline for Delta, asked Gillette to cover herself with a blanket as she felt offended by her action. Gillette refused and was ordered off the flight which was to head to New York from Vermont.
Gillette, who has filed a complaint with the Vermont Human Rights Commission, was sitting in a window seat in the back of the plane and next to her husband when the incident took place, her attorney Elizabeth Boepple, told AFP.
"She was being very discreet in the way she was nursing, her breast was covered, she wasn't flaunting anything and no one else could have seen her nursing her child," Boepple said. "To have her civil right violated like that in such a blatant, flagrant fashion is outrageous."
She said Gillette was heartened by the display of support and had taken part in the protest on Tuesday at the Albuquerque airport in New Mexico.
Freedom Airlines has publicly apologised for the incident and said the flight attendant involved had been disciplined.
"Delta fully supports a mother's right to breastfeed her baby on board our aircraft and we regret the decision to remove the passenger from Flight 6160," Betsy Talton, a spokesperson for Delta, told AFP.
Organisers of Tuesday's "nurse-in", however, said they want the airline to offer Gillette a personal apology.